Reproduction Abstracts

Previous studies have assessed the effects of melatonin on cultured granulosa cells under 5% CO2 in air, and focused on its antioxidant properties. Effects of melatonin under more physiological oxygen tensions are not known. Here we cultured granulosa cells from antral follicles (4–6 mm diameter) in fibronectin-coated 12-well plates (at 600 000 cell/ml) in TCM199, with melatonin added at one of four concentrations (0, 20, 200, 2000 pg/ml) under either i) low ox...

Heat stress can impair ovarian function and reduce fertility in cattle. It is correlated with oxidative stress and can induce granulosa cell apoptosis and reduce steroid production. Effects of heat stress can be reversed by melatonin, but studies with ovarian cells have only been evaluated under atmospheric oxygen tension. Here we cultured granulosa cells (GCs) from antral follicles in fibronectin-coated 12-well plates in M199 for up to 144h under physiological (5%) oxygen ten...